Salmonella enterica serovar Typhi genomic regions involved in low pH resistance and in invasion and replication in human macrophages

Abstract Purpose Salmonella enterica serovar Typhi, the etiological agent of typhoid fever, causes a systemic life-threatening disease. To carry out successful infection process, this bacterium needs to survive alkaline and acid pH conditions presented in mouth, stomach, small intestine, gallbladder. Therefore, work, genetic screening identify S . Typhi genes involved circumneutral resistance was performed. Methods A collection S. mutants deleted fragments ranging from 6 80 kb were obtained by Datsenko Wanner method. Bacterial growth rate assays each mutant performed resistance. deficient at specific evaluated their capacity invade replicate phagocytic cells. Results In it is reported that ∆F4 (pH 4.5), ∆F44 4.5, 5.5, 6.5), ∆F73 6.5, 7.5) grow indicated. These three strains also affected ability human macrophages. Conclusions contains defined genomic regions influence survival values, as well invasion replication inside Thus, information probably allows bacteria different compartments for an efficient cycle.